(2022) Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh. The predominant genotype could be utilized as the future vaccine candidate or vaccines with the dominant genotype should be used to control BRV diarrhea in Bangladesh.Ĭitation: Uddin Ahmed N, Khair A, Hassan J, Khan MAHNA, Rahman AKMA, Hoque W, et al. To control disease, high-risk areas and younger crossbred calves should be targeted for surveillance and management. About 23% of calf diarrhea cases were associated with BRV. The most frequently identified BRV genotype was G6P. The VP4 gene of all P strains showed >90% identities to each other and also with Indian strains. The BRV G6 strains were found to be closest (98.9–99.9%) to Indian strains, and BRV G10 strains showed 99.9% identities with Indian strain. G6P was the predominant genotype (94.4%), followed by G10P (5.6%). Moreover, the risk of BRV infection was 2.6-times (95% CI:1.1–5.8) higher in crossbred (Holstein Friesian, Shahiwal) than indigenous calves. The risk of BRV infection was 3.1-times (95% CI: 1.5–6.5) higher in calves aged ≤ 5 weeks than those aged >5 weeks. The odds of BRV infection were 3.8- (95% confidence interval : 1.0–14.7) and 3.9-times (95% CI:1.1–14.2) higher in Barisal and Madaripur districts, respectively, than Sirrajganj. About 23% of the diarrheic calves were BRV positive. A phylogenetic tree was constructed based on the neighbor-joining method using CLC sequence viewer 8.0. The G and P genotypes of BRV were determined by RT-PCR and sequencing. Risk factors were identified by multivariable logistic regression analysis. The RTSBK positive samples were further tested by polyacrylamide gel electrophoresis and the silver staining technique to detect rotavirus dsRNA. These samples were screened to detect the presence of BRV using rapid test-strips BIO K 152 (RTSBK). Fecal samples were collected from 200 diarrheic calves in three districts between January 2014 and October 2015.
In this study we aimed to identify risk factors for BRV infection and determine the G and P genotypes of BRV strains in diarrheic calves.
#Clc sequence viewer 8 download software
Welcome to CLC Sequence Viewer 7.6.1 - a software package supporting your daily bioinformatics work.Bovine rotavirus (BRV) is considered the leading cause of calf diarrhea worldwide, including Bangladesh. 1.5 When the program is installed: Getting started 1.5.1 Quick start. 1.2.5 Installation on Linux with an RPM-package 1.3 System requirements. 1.2.4 Installation on Linux with an installer. Introduction to CLC Sequence Viewer Contents 1.1 1.2Ĭontact information. 208ĭ Restriction enzymes database configuration 182 16 Phylogenetic treesġ6.1 Phylogenetic tree features. 180 15.4 Bioinformatics explained: Multiple alignments. 172 15 Sequence alignmentġ5.1 Create an alignment. 163 14.2 Restriction site analysis from the Toolbox. 160 14 Restriction site analysesġ4.1 Dynamic restriction sites. 157 13.5 Translation of DNA or RNA to protein. 155 13.3 Reverse complements of sequences. 153 13 Nucleotide analysesġ3.1 Convert DNA to RNA. 139 12 General sequence analysesġ2.1 Extract sequences. 137 10.5 Tools for linking sequence and structure. 129 10.4 Snapshots of the molecule visualization. 127 10.2 Viewing molecular structures in 3D. 123ġ0.1 Importing molecule structure files. Selecting which part of the view to print.
When the program is installed: Getting started. QIAGEN Aarhus A/S Silkeborgvej 2 Prismet DK-8000 Aarhus C DenmarkĬontact information.
#Clc sequence viewer 8 download mac os x
CLC Sequence Viewer 7.6.1 Windows, Mac OS X and Linux SeptemThis software is for research purposes only.
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